Cloning the gene or gene fragment into a pgex expression vector. Bacterial vector for expressing gst fusion proteins with a thrombin site. Sticky ends from different bfuai sites may not be compatible. Screening pgex recombinants for fusion protein expression. Figure 7c shows pgexcs2 which was a modification of pgexcs1.
Weve made finding the right vector for your research easier. This is a free resource for the scientific community that is compiled by addgene. Efficient cleavage requires at least two copies of the bfuai recognition sequence. Handling the vector should be removed from the driice packaging and stored at 20c. This page is informational only this vector is not available from addgene please contact the manufacturer for further details. To see this sequence with restriction sites, features, and translations, please.
Gst gene fusion system the wolfson centre for applied. An expression vector has features that any vector may have, such as an origin of replication, a selectable marker, and a suitable site for the insertion of a. Can anyone help me with iptg induction in pgex4t1 clone. Bfuai is typically used at 50c, but is 50% active at 37c. Vector database is a digital collection of vector backbones assembled from publications and commercially available sources. Find the right expression vector for your orf clone from our comprehensive list of vectors. Do not use internally or externally in humans or animals. Methods employing a plant virus proteinase to cleave expressed fusion proteins are. Also disclosed are recombinant vectors useful for overproducing plant virus. Not recommended or intended for diagnosis of disease in humans or animals. Can anyone help me with iptg induction in pgex 4t1 clone.
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